What to do to show the negative of albuminuria

summary

We know that urinary protein is one of the important items in clinical examination of renal function. Each function plays a great role in the human body. Let's learn what to do to show the negative of proteinuria.

What to do to show the negative of albuminuria

This method is commonly used in hospital laboratory. Preparation of reagent: weigh 3 g of sulfosalicylic acid, add distilled water to 100 ml to prepare 3% sulfosalicylic acid solution. The prepared solution should be stored in a brown bottle and stored in a dark place without sunlight to avoid failure. Test method: take a clean glass test tube, add about 2 ml urine, and then use a dropper to add 3-5 drops of reagent to the urine to observe whether there is protein.

Heating method: take a glass tube, pour the urine to two-thirds of the place, add a few drops of 2% cool acid or vinegar, hold the bottom of the tube obliquely with your thumb and good finger, put it on the flame (such as sprinkling lamp) and directly heat the urine at the upper end of the tube, often turn the tube until the upper section boils, observe whether there is turbidity or precipitation coagulation, and judge the plus sign according to the degree as follows. In order to rule out the false-positive that the turbidity is not caused by protein, 2-3 drops of acetic acid or 10 drops of vinegar should be added. If the turbidity does not disappear at this time, it is a positive reaction, indicating that it is protein.

Test paper method: test paper is a kind of test paper made by reagent factory in advance for urine protein qualitative test, which can be purchased in pharmaceutical stores. The test paper was soaked with reagent and showed blue when it met protein. A standard color plate was attached to the test paper. The method is relatively simple. Take a strip of test paper, immerse it in the urine, take it out immediately, about 10-20 minutes, and observe whether there is blue display. If there is no change for negative, blue, that is, the color; alkaline urine can appear false positive, therefore, before the test, use litmus test paper to check the pH of urine, if it is alkaline (pH 7.0) above), should first add a few drops of acetic acid, make urine acid must do.

matters needing attention

The above three methods are qualitative examination of urinary protein, which only shows whether there is protein in urine and the relative amount, but can not tell the exact amount. If you want to know how much protein is excreted every day, you can do quantitative determination. You need to leave 24 hours of urine for protein quantitative determination.